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| Acceso al texto completo restringido a Biblioteca INIA Tacuarembó. Por información adicional contacte bibliotb@tb.inia.org.uy. |
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Biblioteca (s) : |
INIA Tacuarembó. |
Fecha : |
30/05/2019 |
Actualizado : |
04/05/2020 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
WALLACE, F.; BENNADJI, Z.; FERREIRA, F.; OLIVARO, C. |
Afiliación : |
FEDERICO WALLACE, Espacio de Ciencia y Tecnología Química, Centro Universitario de Tacuarembó, UdelaR, Tacuarembó, Uruguay.; ZOHRA BENNADJI SOUALHIA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FERNANDO AMARY FERREIRA CHIESA, Espacio de Ciencia y Tecnología Química, Centro Universitario de Tacuarembó, UdelaR, Tacuarembó, Uruguay; Laboratorio de Carbohidratos y Glicoconjugados, Departamento de Química Orgánica, Facultad de Química, Udelar, Instituto de Higiene, Uruguay.; CRISTINA OLIVARO, Espacio de Ciencia y Tecnología Química, Centro Universitario de Tacuarembó, UdelaR, Tacuarembó, Uruguay. |
Título : |
Structural characterisation of new immunoadjuvant saponins from leaves and the first study of saponins from the bark of Quillaja brasiliensis by liquid chromatography electrospray ionisation ion trap mass spectrometry.
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Fecha de publicación : |
2019 |
Fuente / Imprenta : |
Phytochemical Analysis, 1 November 2019, Volume 30, Issue 6, Pages 644-652. Doi: https://doi.org/10.1002/pca.2837 |
DOI : |
10.1002/pca.2837 |
Idioma : |
Inglés |
Notas : |
Article history: Received: 19 December 2018; Revised: 21 February 2019; Accepted: 27 March 2019. ACKNOWLEDGEMENTS: This work was supported by the INIA (award L4?FO?21?0?00);
the MEC (Uruguay, Fondo Vaz Ferreira fvf_2017_158) and the PEDECIBA-Química. |
Contenido : |
Abstract. Introduction: Quillaja brasiliensis (St. A. ?Hil. & Tul) Mart (Quillajaceae) is a species native to South America, which is rich in saponins. Saponins are used in different industries, so there is a constant demand for this type of compound. Based on the wide range of applications for the saponins found in this species, notably as
immunoadjuvants, we conducted a comprehensive study of this tree and its saponins. Objective: The purpose of this work is to complete the characterisation of
the immunoadjuvant saponin fraction from Q. brasiliensis leaves and further study the saponin fraction obtained from Q. brasiliensis bark. Methodology: Saponin fractions were studied using mass spectrometry in combination with classical methods of monosaccharide and methylation analysis. We performed direct infusion and liquid chromatography/electrospray ionisation ion trap multiple?stage mass spectrometry (DI?ESI?IT?MSn and LC?ESI?IT?MS2). Results: Seventy?five saponins, 21 from leaves and 54 from bark, were tentatively identified according to their molecular mass, fragmentation pattern and chromatographic behaviour. This work represents the first investigation of saponins from the bark of Q. brasiliensis and some of them presented new structural motifs not previously reported in the genus Quillaja. Conclusion: The efficiency and selectivity of the data dependent LC?MS2 method allowed the rapid profiling of saponins from Q. brasiliensis. The results of the monosaccharide and methylation analysis performed in saponins from Q. brasiliensis fractions and Q. saponaria Molina (Quillajaceae) fraction gives further support to the structures proposed according to the mass spectral data, validating the strategy used in the present work. MenosAbstract. Introduction: Quillaja brasiliensis (St. A. ?Hil. & Tul) Mart (Quillajaceae) is a species native to South America, which is rich in saponins. Saponins are used in different industries, so there is a constant demand for this type of compound. Based on the wide range of applications for the saponins found in this species, notably as
immunoadjuvants, we conducted a comprehensive study of this tree and its saponins. Objective: The purpose of this work is to complete the characterisation of
the immunoadjuvant saponin fraction from Q. brasiliensis leaves and further study the saponin fraction obtained from Q. brasiliensis bark. Methodology: Saponin fractions were studied using mass spectrometry in combination with classical methods of monosaccharide and methylation analysis. We performed direct infusion and liquid chromatography/electrospray ionisation ion trap multiple?stage mass spectrometry (DI?ESI?IT?MSn and LC?ESI?IT?MS2). Results: Seventy?five saponins, 21 from leaves and 54 from bark, were tentatively identified according to their molecular mass, fragmentation pattern and chromatographic behaviour. This work represents the first investigation of saponins from the bark of Q. brasiliensis and some of them presented new structural motifs not previously reported in the genus Quillaja. Conclusion: The efficiency and selectivity of the data dependent LC?MS2 method allowed the rapid profiling of saponins from Q. brasiliensis. The results of the monosaccharide and methyla... Presentar Todo |
Palabras claves : |
IMMUNOADJUVANT SAPONINS; MASS SPECTROMETRY; METHYLATION ANALYSIS; MONOSACCHARIDE ANALYIS; QUILLAJA BRASILIENSIS. |
Asunto categoría : |
K11 Ingeniería forestal |
Marc : |
LEADER 02910naa a2200241 a 4500 001 1059792 005 2020-05-04 008 2019 bl uuuu u00u1 u #d 024 7 $a10.1002/pca.2837$2DOI 100 1 $aWALLACE, F. 245 $aStructural characterisation of new immunoadjuvant saponins from leaves and the first study of saponins from the bark of Quillaja brasiliensis by liquid chromatography electrospray ionisation ion trap mass spectrometry. .$h[electronic resource] 260 $c2019 500 $aArticle history: Received: 19 December 2018; Revised: 21 February 2019; Accepted: 27 March 2019. ACKNOWLEDGEMENTS: This work was supported by the INIA (award L4?FO?21?0?00); the MEC (Uruguay, Fondo Vaz Ferreira fvf_2017_158) and the PEDECIBA-Química. 520 $aAbstract. Introduction: Quillaja brasiliensis (St. A. ?Hil. & Tul) Mart (Quillajaceae) is a species native to South America, which is rich in saponins. Saponins are used in different industries, so there is a constant demand for this type of compound. Based on the wide range of applications for the saponins found in this species, notably as immunoadjuvants, we conducted a comprehensive study of this tree and its saponins. Objective: The purpose of this work is to complete the characterisation of the immunoadjuvant saponin fraction from Q. brasiliensis leaves and further study the saponin fraction obtained from Q. brasiliensis bark. Methodology: Saponin fractions were studied using mass spectrometry in combination with classical methods of monosaccharide and methylation analysis. We performed direct infusion and liquid chromatography/electrospray ionisation ion trap multiple?stage mass spectrometry (DI?ESI?IT?MSn and LC?ESI?IT?MS2). Results: Seventy?five saponins, 21 from leaves and 54 from bark, were tentatively identified according to their molecular mass, fragmentation pattern and chromatographic behaviour. This work represents the first investigation of saponins from the bark of Q. brasiliensis and some of them presented new structural motifs not previously reported in the genus Quillaja. Conclusion: The efficiency and selectivity of the data dependent LC?MS2 method allowed the rapid profiling of saponins from Q. brasiliensis. The results of the monosaccharide and methylation analysis performed in saponins from Q. brasiliensis fractions and Q. saponaria Molina (Quillajaceae) fraction gives further support to the structures proposed according to the mass spectral data, validating the strategy used in the present work. 653 $aIMMUNOADJUVANT SAPONINS 653 $aMASS SPECTROMETRY 653 $aMETHYLATION ANALYSIS 653 $aMONOSACCHARIDE ANALYIS 653 $aQUILLAJA BRASILIENSIS 700 1 $aBENNADJI, Z. 700 1 $aFERREIRA, F. 700 1 $aOLIVARO, C. 773 $tPhytochemical Analysis, 1 November 2019, Volume 30, Issue 6, Pages 644-652. Doi: https://doi.org/10.1002/pca.2837
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| Acceso al texto completo restringido a Biblioteca INIA La Estanzuela. Por información adicional contacte bib_le@inia.org.uy. |
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Biblioteca (s) : |
INIA La Estanzuela. |
Fecha actual : |
02/03/2017 |
Actualizado : |
03/03/2017 |
Tipo de producción científica : |
Abstracts/Resúmenes |
Autor : |
CAFFARENA, D.; GIANNITTI, F.; SCHILD, C.; CASAUX, M.; FRAGA, M.; RIET-CORREA, F. |
Afiliación : |
RUBEN DARÍO CAFFARENA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FEDERICO GIANNITTI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; CARLOS SCHILD, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARÍA CASAUX, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARTIN FRAGA COTELO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FRANKLIN RIET-CORREA AMARAL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
Título : |
Brote de actinobacilosis cutanea y ganglionar en bovinos lecheros./Outbreak of cutaneous and ganglionar actinobacillosis in dairy cattle. |
Fecha de publicación : |
2016 |
Fuente / Imprenta : |
In: SEMINARIO DE LA FUNDACIÓN "CHARLES LUIS DAVIS" EN ARGENTINA ,10o., REUNIÓN ARGENTINA DE PATOLOGÍA VETERINARIA, 10, (RAPAVE), Esperanza, Santa Fe, AR: Facultad de Ciencias Agrarias y Veterinarias. Agosto 24-26 2016. |
Serie : |
(Revista de Medicina Veterinaria, Bs.As, 2016, v. 97 n.3, p.28). |
Idioma : |
Español |
Thesagro : |
ENFERMEDADES DE LOS ANIMALES. |
Asunto categoría : |
-- |
Marc : |
LEADER 00844nam a2200181 a 4500 001 1056755 005 2017-03-03 008 2016 bl uuuu u01u1 u #d 100 1 $aCAFFARENA, D. 245 $aBrote de actinobacilosis cutanea y ganglionar en bovinos lecheros./Outbreak of cutaneous and ganglionar actinobacillosis in dairy cattle.$h[electronic resource] 260 $aIn: SEMINARIO DE LA FUNDACIÓN "CHARLES LUIS DAVIS" EN ARGENTINA ,10o., REUNIÓN ARGENTINA DE PATOLOGÍA VETERINARIA, 10, (RAPAVE), Esperanza, Santa Fe, AR: Facultad de Ciencias Agrarias y Veterinarias. Agosto 24-26 2016.$c2016 490 $a(Revista de Medicina Veterinaria, Bs.As, 2016, v. 97 n.3, p.28). 650 $aENFERMEDADES DE LOS ANIMALES 700 1 $aGIANNITTI, F. 700 1 $aSCHILD, C. 700 1 $aCASAUX, M. 700 1 $aFRAGA, M. 700 1 $aRIET-CORREA, F.
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